Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Optical absorbance. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Table of Contents. SpectraMax® iD5. 2. edu) to be added as a trained user in iLab. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. l. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. both cuvette-based and microplate reader applications on the same instrument. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Microplate Readers include the M3, M4, M5, and M5e models. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. To block unwanted residual excitation light and minimize background interference, a 570 nm. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 1** Version 7. SDS-PAGE Assay. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Learn how you can utilize microplate readers to drive landmark discoveries. 5 Dual-luciferase reporter assay. Fill a clean plate with water. 00 USD. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 4. SpectraMax M5e Multi-Mode Microplate Reader. Click SoftMax Pro Software User Guide. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Fluorescence Microplate Readers. The SpectraMax M2 and M2e are versatile and reliable. At the end of this period, cells were exposed to FA (1 mM) for 1 h. The spectrophotometric. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Microplate Readers are modular and . Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. TR-FRET assay principle. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Wide range of applications. SpectraMax® Aquamax. Published Dec 16, 2020 | Updated Dec 16, 2020. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Fifteen different automated tests are provided. book Page 7 Wednesday, July 7, 2010 2:09 PM. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Microplate Readers Biotek Synergy H1 Hybrid Microplate. Microplate Readers are modular and . Bookings are free, but you will need to use iLab to book. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The following document is. Between steps, reactions were rinsed 3 times with TBS + 0. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. For the Live/Dead assay, they were treated. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. TR-FRET assay principle. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 1. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Figure 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 6 for further experiment, so I'm confused which i would choose for OD value determination. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. 00 USD. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 7°C (gradient heating design). These lab workhorses are used in drug discovery, research, bioassay validation, and. 314 low lamp level warning. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 6 0. No additional manuals are available. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Make sure the microplate is flat against. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The assay applies fluorescence polarization to quantify Fc-containing IgG. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. 5°C at 37°C. 313 reference gain check fail. Molecular Devices SpectraMax® series microplate readers. Cavicide (Warehouse, Cat # 79300360). The assay window spanned four orders of magnitude. Retrieving Each. book Page 7 Wednesday, July 7, 2010 2:09 PM. Choose from a. 9000-0756. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. The integration time suggested is 5-10 sec. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. SpectraMax M5 Multi-Mode Microplate Reader. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 0 . SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Compact and tunable absorbance microplate readers DATA SHEET. book Page 7 Wednesday, July 7, 2010 2:09 PM. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax M Series multi-mode microplates are configurable readers with triple. Add to Cart. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. 2. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The CellTiter-Glo assay from Promega uses luciferase. • Tel. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. 1. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. We would like to show you a description here but the site won’t allow us. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. All associated StakMax® stackers. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. e. The PBS solution (10. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. SpectraMax® ABS. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. The dual monochromator optics allow the. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. TR-FRET assay. Published Dec 16, 2020 | Updated Dec 16, 2020. reader in the visible range between 405–750 nm with NIST and NMI. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 96-384. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Download PDF. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 14 Average %CV 0. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. View the Molecular Devices website. FlexStation™ I. Equipped with scanning, shaking and kinetics modules. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Ferguson, Ph. Page 1 of 8 . TR-FRET assay. Self Service Available. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The dual monochromator optics allow the widest range of applications to be utilized for bior. Western blotting assay. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. 1 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The cuvette must be in place when you read the plate. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The Spectr. • Tel. Lower limits of detection for ATP determined using the ATPlite . 72 MB. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 1. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). Figure 4: Transcreener ADP. The Multiskan Sky microplate reader is an absorbance plate reader. Table of Contents. AquaMax® 4000. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Wide range of applications The VersaMax reader covers. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Figure 1. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. This application note describes how to. 72 MB. FlexStation™ III. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Figure 1. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. AquaMax® 4000. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Molecular Devices SpectraMax® series microplate readers. l. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. System Diagrams and Dimensions. Recertification is recommended every two years. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Here the delta mP was 336, and Z’ factor was 0. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. 87. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 5 12. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. For the Live/Dead assay, they were treated. Shop now. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. 7°C (gradient heating design). TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Detailed specifications of each model reader are reported in Section 7, “Specifications”. In . This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Five preferred detection modes. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. 96-1536. 4 Max StDev 1. 2. The samples were boiled for 5 min with 1 ×. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. 1. 2. SpectraMax® M5. 26 1. 54 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 6: bioNova científica, s. 1. Linear, Orbital Slow, and Orbital Fast. Western blotting assays were conducted according to previous studies . The cells were then treated to induce apoptosis. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. When a feature or capability applies to only certain readers, this exception is noted. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax® M4. 2. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. No accessories are needed and SoftMax® Pro Software. 1. The guide also covers the software features, the specifications, and the accessories of the systems. 0 OD for earlier models. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Table of Contents. When a FlexStation® 3 Multi-Mode Microplate Reader. Filterless readers provide consistent results with steady temperature regulation. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 1. Standard features include a. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Cuvette port functionality can also be controlled using SoftMax Pro Software. STAKMAX. Microbiota DNA was. 1 second. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. used. The. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Fluorescence Microplate Reader Comparison Chart. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 16S rRNA gene sequencing . This reader's dual monochromators for. Download PDF. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. 40266 Email: drugdiscoverytech@lifetech. Softmax Pro GxP Software 6. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax Spectrofluorometers. Or scan up to 384 wells at any time to determine the best assay conditions. SpectraMax® Aquamax. Figure 1. edu) to be added as a trained user in iLab. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 6 for further experiment, so I'm confused which i would choose for OD value determination. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Parallel negative control assays had primary, secondary, or both antibodies omitted. ,. The following document is intended to demonstrate setup of this instrument. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 1. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Wide range of applications. SpectraMax® M5e. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Offer Ends In: 44 Days 10: 22: 56. Please refer to the Obsolete Microplate Reader Models for more information. 7 1. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The advantage of using labeled probes is detection specificity. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Here the delta mP was 336, and Z’ factor was 0. Molecular Devices | Life Science Applications | Bioanalytical. 0 μL) instead of the enzyme solution was used as the sample blank group. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Examples: “Press carefully to engage,” “ Do not. #SpectraMax L Config) Methods TIGIT/CD155 Blockade Bioassay. 2. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. M3-M5e_manual. 4. es •. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. SpectraMax Glo Steady-Luc Reporter Assay Kit. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 7°C and the bottom to 36. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. However, when a specific probe is not available, or it causes off-target effects on the. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Description. The spectrophotometric. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. ATP standard curves read on SpectraMax microplate readers. Five preferred detection modes. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. View the Molecular Devices website. M3-M5e_manual. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Nontreated cells in DMEM medium were used as a control. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. View the Molecular Devices website. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The calculated LOQ values were 0. Table 1. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. 58% for the detection module and 2. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Introduction. No filter change with dual monochromator optics. As. The system. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options.